Protran Blotting Membranes

• Best and easiest blocking of unspecific binding compared to all blotting membranes
• Superior for chemiluminescence detection using horseradish peroxidase based systems
• Unsupported, 100 % pure nitrocellulose membrane

• Reinforced nitrocellulose membranes
• Allows multiple reprobings without loss of membrane integrity
• Excellent signal-to-noise ratio

Protran^® nitrocellulose membranes are the most frequently specified transfer media in the world for Western, Southern and Northern blotting. Additional the membrane is excellent for carbohydrate studies (Glycomics).
Protran membranes are manufactured using 100 % pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as “nitrocellulose“ may actually contain large amounts of cellulose acetate which will lower the protein binding capacity.
Protran^® membranes have the best handling strength of all pure nitrocellulose membranes. They are compatible with a variety of detection methods, including isotopic, chemiluminescent (peroxidase/ luminol-based), colorimetric and fluorescent. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wet in water, and then in the transfer buffer. No other pre-treatment steps are necessary.

High binding capacity
The 0.20 μm pore size of the Protran (NC 0.2) nitrocellulose membrane ensures high binding capacity of small samples below 20 kD by reducing “blow-through“. The 0.45 μm pore size membrane (NC 0.45) is ideal for larger molecular weight samples.

Low background
In addition to the high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signaltonoise ratios, without the need for stringent washing conditions.

Protein stability for years
A particular benefit of the proprietary Protran nitrocellulose formula is the proven excellent shelf life of proteins. Empirical evidence shows that proteins maintain molecular recognition activity for five years on Protran, which makes it the standard for protein blotting.

The Protran supported^®
membrane consists of pure 100 % nitrocellulose cast onto both sides of an inert polyester support material. The support in no way affects transfer conditions or results and gives the membrane exceptional handling characteristics, allowing it to be reprobed repeatedly.

The Protran supported^® nitrocellulose membrane provides high sensitivity with very low non-specific binding without stringent washing and blocking conditions using standard nitrocellulose protocols.

The combination of flexibility, strength and excellent signal-to-noise ratios makes the Protran supported^® membrane ideal, especially when procedures involve repeated stripping and reprobing.

Protran supported^®  supported nitrocellulose membranes combine sensitivity, strength and savings.

Membrane type

Nitrocellulose, 100 % pure

Nitrocellulose, reinforced

Binding capacity

80 – 90 μg/cm²

Approx. 60 - 80 μg/cm²

Pore sizes

0.45 μm
0.20 μm

0.45 μm

Semi-dry blotting

++

++

Tank blotting

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++

Vakuum blotting

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++

Capillary blotting

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++

Drying

Proteins

Proteins

UV-crosslinking

DNA and RNA

DNA and RNA

Backen (80 °C)

DNA und RNA

DNA und RNA

Colorimetric

++

++

Chemiluminescent*

++

++

Isotopic

++

++

Fluorescent

Proteins

–

846-B10401396

Protran
NC 0.2

0.20 µm

30 cm x
4 m

1 roll

846-B10401196

Protran NC 0.45

0.45 µm

30 cm x
4 m

1 roll

846-B10401180

Protran NC 0.45

0.45 µm

30 cm x 60 cm

5 sheets/pack

846-B10402580
on request (discontinued)

Protran NC 0.45

0.45 µm

33 cm x 56 cm

5 sheets/pack

846-B10439196

Protran
supported NC 0.45

0.45 µm

30 cm x 4 m

1 roll